LC/MS Analysis of Nucleic Acid-Related Compounds
In the example introduced here, nucleic acid-related compounds were analyzed by LC/MS, which offers mass information and enables high-sensitivity analysis.
Fig. 1 shows the structure and ESI mass spectrum of the purine base adenine and of the nucleotide adenosine. Under acidic conditions in the positive ion mode, the protonated molecule [M+H]+ is observed as a reference peak.
Fig. 2 shows the LC/MS analysis results of a standard mixture of nucleic acids. SIM measurements were conducted using [M+H]+ as the detected ion for each nucleic acid. Satisfactory separation of the 15 components was achieved.
• The newly developed ion optical system, Qarray® Optics, achieves superior sensitivity, reproducibility, and linearity.
• Rapid, 15,000 u/sec scanning achieves high ion permeability while maintaining the resolution.
• The LCMS-2020 dramatically enhances long-term stability and ease of maintenance.
This page may contain references to products that are not available in your country.
Please contact us to check the availability of these products in your country.