Mouse and Plant Genotyping Using FTA® Cards
Metabolite Analysis
Mouse and Plant Genotyping Using FTA® Cards
Simple PCR was performed using a combination of Ampdirect® to eliminate DNA purification and FTA® Cards manufactured by Whatman. This combination significantly reduces the effort required for genotyping of knockout mice and transgenic mice and for plant genotyping, and also the DNA sample san be stored on the card for a long period at room temperature.
Test Flow
Gene Amplification Reagent Ampdirect®
- DNA present in blood can be amplified directly.
- No DNA extraction is required.
- Thus, risk of cross contamination and/or procedural error is dramatically reduced.
- Only small volumes of blood are necessary (0.5-1μl ).
- Various anticoagulants (including sodium citrate, dipotassium EDTA, and sodium heparinate) can be used.
- Blood (citrated or EDTA-treated blood is recommended) stored frozen for long periods can be analyzed.
- Simplifies PCR methods resulting in its use in a broad range of routine applications including mass screening.
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