Shimadzu Review Vol.80[3・4](2023)
Diagnostics

SPECIALLY COLLECTED PAPERS

Development of Cultivation Optimization Assistance Software

by Shuhei Yamamto1Kento Motoyama, Ph.D.2Kaori Yamada2Taro Nishikawa3Hiroaki Tsushima3Taku Tsuzuki4Tatsuki Higashi4Yosuke Ozawa, Ph.D.4Takashi Suzuki, Ph.D.1Toru Ezure, Ph.D.1

Shimadzu Review 80[3・4] (2023)

Abstract

Shimadzu has developed a system for simultaneously analyzing culture components and metabolites contained in culture supernatant using high-performance liquid chromatograph mass spectrometers (LC-MS). This system enables the analysis of 144 components in culture supernatants, including amino acids and nucleic acid-related compounds, in just 20 minutes per sample. The information obtained, such as about the depletion of culture medium components, is valuable for optimizing culture conditions.
However, one remaining challenge was that the system was still dependent on trial and error processes by users to interpret the vast amounts of data acquired and evaluate the culture conditions required to ensure efficient cultivation. To address that challenge, joint development was initiated with Epistra Inc., which has expertise in AI-based optimiza-tion of cultivation. Currently, advancements toward the development of a software product for supporting data-driven optimization of cultivation conditions are being achieved using an Epistra optimization engine. Using that product, validation experiments were conducted for optimizing cultivation in the production of CHO cell antibodies. Four rounds of experimental condition generation using AI achieved an approximately 40% increase in antibody concentration compared to the control.
This article describes an overview of the software in development and details of the validation experiments.


1Cell Business Unit, Diagnostics Business Development, Analytical & Measuring Instruments Division, Shimadzu Corporation, Kyoto, Japan
2Biology・Chemistry Unit, Technology Research Laboratory, Shimadzu Corporation, Kyoto, Japan
3Research & Development Department, Analytical & Measuring Instruments Division, Shimadzu Corporation, Kyoto, Japan
4Epistra Inc., Tokyo, Japan

*The information contained in Shimadzu Review has not been modified since the original publication date. Please be aware that in some cases, products mentioned within the articles are no longer available.