High-Speed and High Sensitivity Analysis of Active Ingredients in Mouthwash

User Benefits

- Enables high sensitivity analysis of three types of active ingredients within 4 minutes. - Basic compounds such as quaternary ammonium salts can be analyzed with good repeatability without peak tailing. - The photodiode array (PDA) detector allows chromatograms of any wavelength to be obtained with a single analysis.

Introduction

Commercial oral care products (mouthwashes) contain multiple active ingredients, which are generally analyzed by HPLC. When the quaternary ammonium salt CPC (cetylpyridinium chloride) is analyzed with a C18 column, interactions between the CPC and residual silanol groups remaining on the surface of the silica gel packing material are known to cause adsorption and peak tailing. Application News No. 01-00116A describes high-speed and simultaneous analysis of active ingredients CPC and GK2 (dipotassium glycyrrhizinate) in mouthwash using a Shim-pack Arata C18 column. It was shown that using that column, satisfactory peak shapes were obtained without addiing an ion-pairing reagent or perchlorate to an acidic mobile phase. Furthermore, if high sensitivity analysis is desired, it is effective to use a mobile phase with low UV absorption. This article describes high-speed and high sensitivity analysis of CPC, GK2, and isopropyl methylphenol (IPMP) in mouthwash using a phosphate buffer-based mobile phase.

May 23, 2023 GMT