Establishment of a Method for Direct Analysis of the Mouse Liver Metabolome Using the DPiMS™-8060
In the analysis of endogenous metabolites (metabolome analysis), it is difficult to perfectly remove biases caused by pretreatment and sampling. Therefore, in order to accurately grasp the changes in the metabolome of a biospecimen, the establishment of a method for direct analysis of the metabolome is indispensable. This article introduces a method established to directly analyze the metabolome of a tissue sample (intact metabolome analysis) using a PESI tandem mass spectrometer together with the application of the method to the metabolome analysis of CCl4-induced acute liver failure model mice.
- Content Type:
- Application
- Document Number:
- LAAN-A-TM-E059
- Product Type:
- Liquid Chromatograph-Mass Spectrometry, Mass Spectrometry
- Keywords:
- PESI, DPiMS, Life science, Metabolomics, Metabolome, Metabolite, Direct analysis, Rapid analysis, Mouse, Liver, Amino acid, Lipid, Sugar, Multivariate analysis, Clinical research, Forensics, Pharmaceutical, Life Science, DMPK, ADME, Safety testing, Development (Formulation, Scale-up, Method development), DPiMS-8060
- Language:
- English
- File Name:
- jpo119005
- File Size:
- 276kb
