Q: How fast should I set the mobile phase flow rate when using a high-speed analysis column?
A:
In general, consideration of the Van Deemter equation indicates that using packing consisting of smaller particles improves both the column efficiency and the optimum mobile phase line speed. For the Shim-pack XR-ODS too, with its particle diameter of 2.2 µm, the appropriate mobile phase flow rate is higher than it is for a column with a particle diameter of 5 µm. The table indicates the optimum mobile phase flow rate ranges for all the different inner diameters of Shim-pack XR-ODS columns and so set the mobile phase flow rate after referring to this table and considering the pressure resistance of the system and column.
