Culture Medium Analysis for a Metabolic Analysis of Antibody-Producing CellsUsing LC-MS/MS and ICP-MS

User Benefits

- Performs simultaneous measurement of organic components and inorganic components (separately) in culture medium and culture supernatant with simple pretreatment. - Performs a metabolic analysis that includes both organic and inorganic components.

Introduction

Antibody drugs are produced by culturing antibody-producing cells. Developing these host cells is an active field of research and the Omasa Laboratory at Osaka University recently established a cell line derived from Chinese hamster lung cells (CHL-YN cells) . CHL-YN cells have approximately 2-fold growth rate compared to CHO-K1 cells, a cell line that is frequently used in antibody production. Understanding the metabolism of host cells is important to realize industrial applications of these cells, but the metabolism of CHL-YN cells has not yet been fully studied. Recent studies have reported the effects of changes of organic/inorganic components in the culture medium on antibody production and quality. Organic and inorganic components are also known to interact with one another in culture medium over the course of antibody production; hence an analysis of the culture medium must include both organic and inorganic components to be able to understand host cell metabolism. Furthermore, since organic/inorganic components are known to interact with each other in the culture medium2); we consider the analysis of both organic and inorganic components in the culture medium is important for understanding metabolism. This Application News uses LC-MS/MS (LCMS-8060NX) to analyze 144 organic compounds and ICP-MS (ICPMS-2030) to analyze 9 inorganic elements in the culture medium and culture supernatant of a CHL-YN antibody producing cell line culture and describes using this data to identify which components and metabolic pathways are involved in antibody production.

May 28, 2023 GMT