Analysis of Long-Chain Amino Acid Sequences Using a Protein Sequencer —Isocratic System —

User Benefits

- Amino acid sequences from the N-terminal can be reliably identified. - Amino acid sequences can be automatically and easily predicted using software. - Even protein amino acid sequences not registered in a genome database can be easily identified.

Introduction

Shimadzu PPSQ-50A series protein sequencers determine amino acid sequences based on Edman degradation. They can identify amino acid sequences in pmol levels of purified proteins. Though samples used for analysis must be purified, the process is extremely simple. For example, after proteins are separated by electrophoresis, they are electrically transferred (electroblotted) onto a PVDF membrane and dyed. Then the protein spots are excised, placed directly in the reactor, and analyzed automatically. This article describes using this system to analyze a long-chain amino acid sequence from the N- terminal.

25 de abril de 2023 GMT