Simultaneous Analysis of 20 Steroid Hormones and High-Sensitivity Analysis of Estrogens

User Benefits

- Simultaneous analysis of 20 steroid hormones in 15 minutes - Derivatization of estrogens for high-sensitivity analysis

Introduction

Steroid hormones are a group of biomolecules characterized by having a steroid skeleton in their molecular structure and are involved in the control mechanisms of a wide range of physiological phenomena, including metabolism, nerve transmission, gene expression, reproduction, blood pressure, and vascular permeability. In recent years, the approach of analyzing multiple steroid hormones simultaneously and analyzing them as profiles has been attracting attention as it has brought new findings in the fields of regenerative medicine and carcinogenesis. The ability to easily measure steroid hormone profiles is expected to accelerate research that will lead to the elucidation of the mechanisms of various diseases and therapeutic methods. Immunoassay techniques, such as ELISA and RIA, are widely used for quantitation of steroid hormones, but they are not suitable for profile measurements because they require reagent kits and sample volumes for each measurement item. Immunoassays also carry the risk of inaccurate results due to antibody cross-reactivity, which has led to the development of highly specific steroid hormone quantification methods using LC/MS/MS for research applications. However, since steroid hormones in biological samples are a mixture of extremely low concentrations of some steroids, such as estrogen, and high concentrations of others, various innovations are required to establish simultaneous analysis even with LC/MS/MS methods that excel in quantitative dynamic range. This article introduces an example of simultaneous determination of 20 steroid hormones in human blank serum matrix using the LC/MS/MS Method Package for Steroid Hormones. This method package includes a sample preparation procedure and analytical conditions that enable the quantification of 20 steroid hormones.

May 25, 2023 GMT