Multi-Charged Ion Analysis of Intact Antibodies Using a Quadrupole Time-of-Flight Mass Spectrometer

User Benefits

- Analysis of intact antibodies can be performed using the LCMS-9030 capable of acquiring accurate mass. - The ReSpect algorithm installed in LabSolutions Insight Explore CSD enables multi-charged ion analysis (charge deconvolution) of macromolecular compounds such as proteins. - The reconstructed zero charge spectrum of glycoform patterns of monoclonal antibodies can be detected with this workflow.

Introduction

In recent years, antibody drugs such as monoclonal antibodies and antibody drug conjugates have been actively researched and developed because of their high specificity for target molecules and fewer side effects. Unlike low molecular drugs, which can be mass-produced by chemical synthesis, antibody drugs are produced using animal cells. Therefore, it is difficult to prevent structural heterogeneity, and characterization is indispensable in the research and development of antibody drugs. Characterization items by mass spectrometry include molecular weight confirmation and glycosylation analysis of intact antibodies. In this article, the analysis of trastuzumab, a monoclonal antibody drug, using the quadrupole time-of-flight mass spectrometer LCMS-9030 is introduced as an example. Multi-charged ion analysis (charge deconvolution) was performed on the obtained mass spectrum using the analysis software LabSolutions Insight Explore CSD.

June 29, 2023 GMT