Identification Analysis of Lactobacillus Species/Strains Using IRXross

User Benefits

- Efficient measurement of multi-specimen is possible by using a diffuse reflectance measurement accessory and microfocus plate. - Since the measurement time with this technique is about 30 seconds per specimen, analysis work can progress in a shorter time than with other techniques. - This technique is also applicable to samples other than bacteria, and enables identification analysis by principal component analysis and cluster analysis.

Introduction

Identification analysis of bacteria is necessary in applied research in the clinical medical field and for quality control in food and drug manufacturing processes. As conventional techniques, the polymerase chain reaction (PCR) method and pulsed-field gel electrophoresis (PFGE) method are widely used, but these methods have various problems, including the complexity of sample preparation and the time required for measurements. The Fourier transform infrared spectrophotometer (FTIR) is an analytical instrument that is applied to a wide range of target samples, and has the potential to become a new technique which solves the above-mentioned problems owing to its simple operation and fast measurement speed. Since the infrared spectrum of bacteria acquired by FTIR contains information originating from lipids, polysaccharides, proteins, carbohydrates and other compounds, it is possible to identify the species and strains of bacteria cultured under the same conditions (culture medium, temperature, time). This Application News article introduces an identification analysis of lactic acid bacteria (lactobacilli) using a combination of an IRXross Fourier transform infrared spectrophotometer and a diffuse reflectance measurement accessory.

July 27, 2023 GMT