Simultaneous Determination of Fat-Soluble Vitamins in Beverages Using Triple Quadrupole LC-MS/MS

User Benefits

- Rapid and simultaneous determination of 13 fat-soluble vitamins A, D, E, and K. - High-sensitivity analysis using a triple quadrupole mass spectrometer. - Individual quantification of α-carotene and β-carotene were achieved.

Introduction

Vitamins play an important role for maintaining human body functions in good condition and need to be obtained from food because they cannot be synthesized in the body. Vitamins A, D, E, and K, which are not soluble in water, are called fat-soluble vitamins. Vitamin A mainly refers to retinol, which has the function of keeping eyes and skin healthy and enhancing immunity. On the other hand, α-carotene and β-carotene are called provitamin A because they are converted to retinol in the body. The conversion rates of those two compounds are different, and the bioavailabilities of α- and β-carotene are estimated to be 1/24 and 1/12 compared to that of retinol, respectively. To evaluate the activity of vitamin A, individual quantification of α- and β-carotene are necessary. It is difficult to separate these compounds with LC-MS/MS in the past since these are isomers and therefore have very similar structures. In this paper, we introduce simultaneous quantification of fat- soluble vitamins including α- and β-carotene by optimizing LC conditions. We performed individual quantification of α- and β- carotene and recovery tests on vegetable juice using this method. As α- and β-carotene do not have ester compounds, alkaline hydrolysis treatment, which is usually needed in sample preparation, can be omitted. Good recovery rates were obtained with a simple extraction operation.

8 de agosto de 2023 GMT