GC/MS and LC/MS Analysis of Metabolites in Hepatitis Model Mouse Serum

User Benefits

- Multi-omics Analysis Package can be used to analyze data obtained by GC/MS and LC/MS measurements. - Multi-omics Analysis Package enables multivariate analysis, including principal component analysis. - Acquired data can be easily visualized using a metabolic map template.

Introduction

Metabolomics is technology used to comprehensively measure all metabolites in living organisms. Metabolomics is used in various fields. In the medical field, it is used to search for biomarkers and determine pathogenesis. A variety of analytical instruments are used to measure metabolites. In particular, chromatographs and mass spectrometers are often used in combination because they can measure various metabolites with high sensitivity. Liquid chromatography/mass spectrometry (LC/MS) and gas chromatography/mass spectrometry (GC/MS) differ in detectable compounds, and both methods can be used to obtain complementary data. This article describes using GC/MS and LC/MS data analysis methods with Multi-omics Analysis Package . As samples, serum was measured from mice treated with a choline-deficient (CD) and a methionine- and choline-deficient (MCD) diet, known as nonalcoholic fatty liver (NAFL) and nonalcoholic steatohepatitis (NASH) models, respectively. By using Multi- omics Analysis Package, GC/MS and LC/MS data were integrated and visualized on a single metabolic map.

5 de octubre de 2023 GMT