High Purity Preparative Purification Enabled by UV/MS Trigger on LC-MS System

User Benefits

- Scaling-up from analytical column to preparative column and purity/recovery check can be completed in a single system. - High identification performance of MS enables high purity fractionation of target compounds. - Utilizing software simulation to optimize parameters for fractionation reduces the labor required.

Introduction

Preparative HPLC is utilized in various fields, such as pharmaceuticals, food, and chemical engineering, for purifying target compounds from mixed samples, searching for active ingredients in natural products, and analyzing the structures of impurities and unknown compounds. In Application News “01- 00650-EN”, an introduction was made to analytical/preparative convertible LC-MS system designed to manage the entire preparative purification workflow. This workflow consists of the optimization of separation conditions at analytical scale, scaling-up, fractionation, and confirmation of purity/recovery. The process began with the efficient optimization of separation conditions, achieved using LabSolutions MD, a dedicated software for supporting method development, to ensure adequate separation between the target compound for fractionation (Hydrocortisone) and the nearby co-eluted peaks. Then followed by loadability optimization, scaling-up, and fractionation with UV signal as a trigger. This article introduces the achievement of higher purity in the fractionation of Hydrocortisone by effectively eliminating impurities through the use of MS signal, which offers enhanced identification performance in addition to UV signal.

21 de noviembre de 2023 GMT