Oligonucleotide analysis using a compact MALDI digital ion trap mass spectrometer MALDImini-1

User Benefits

- Enables molecular weight analysis and sequence analysis of oligonucleotides, including modification sites. - MSⁿ enables reliable end sequence analysis. - The compact benchtop design does not take up much space.

Introduction

Due to rapid progress in practical applications of oligonucleotide therapeutics in recent years, there is a growing need for corresponding analytical technologies. The application of mass spectrometry (MS) to oligonucleotide therapeutics analysis has already begun, but more accurate and rapid analytical techniques are required. Most approved oligonucleotide therapeutics are based on single- or double-stranded synthetic oligonucleotides that are several tens of bases in length (molecular weight of about 6000 to 15000 Da). Such oligonucleotides contain a variety of chemical modifications depending on the purpose. The MALDImini-1 matrix-assisted laser desorption/ionization- digital ion trap-mass spectrometer (MALDI-DIT-MS) is a compact benchtop instrument that can perform MS analysis for m/z values up to 70,000 and MSⁿ analysis for m/z values below 5,000 in positive ion mode. This article presents an example of using the MALDImini-1 system to analyze the molecular weight and sequence of a synthetic oligonucleotide with the same sequence as mipomersen, an oligonucleotide therapeutic.

November 28, 2023 GMT