Qualitative Analysis of Synthetic DNA Using a Single Quadrupole Mass Spectrometer

User Benefits

- Oligonucleotides can be easily analyzed using a Nexera XS inert UHPLC system and an LCMS-2050 single quadrupole mass spectrometer. - The molecular weight of oligonucleotides can be estimated by deconvoluting the obtained mass spectra.

Introduction

Oligonucleotide therapeutics have attracted attention in recent years as a new modality for drug discovery. Typically, they are produced by chemical synthesis, so to ensure product quality it is important to confirm that the synthesized oligonucleotides have the expected base sequence. Mass spectrometry, which provides molecular weight information, is a valuable analytical tool in such cases. This article describes, using an inert UHPLC system and an LCMS-2050 single quadrupole mass spectrometer with user-friendly operability similar to LC systems to analyze the mass of oligonucleotides within a 1 Da margin of error from theoretical values.

December 19, 2023 GMT