Efficient Method Development Using Single Quadrupole Mass Spectrometer ーAutomatic Detection of Co-eluted Peaksー

User Benefits

- LCMS-2050 single quadrupole mass spectrometer provides not only accurate peak tracking based on m/z but also automatic detection of co-eluted peaks for efficient method development. - LabSolutions MD enables easy searching for method operatable design region that satisfies the criteria of resolution for multiple compounds.

Introduction

In the process of separation optimization in LC method development, optimal analytical conditions are searched by varying parameters such as mobile phase composition, gradient curve, and column oven temperature. However, accurate peak tracking among obtained chromatograms is generally challenging due to variations in elution times and co-eluted compounds caused by different analytical conditions through optimization. A PDA (photodiode array) detector can provide peak tracking using differences in UV spectra, but for co-eluted peaks and related substances such as impurities and degraded products, accurate identifications by UV spectra may be difficult. On the other hand, the use of a mass spectrometer in addition to a PDA detector is expected to improve the accuracy of determining co-eluted peaks and tracking related substances based on m/z. This article describes how to improve the efficiency of separation optimization in method development through accurate peak tracking and automatic detection of co-eluted peaks by utilizing LCMS-2050 and LabSolutions MD, a dedicated software for supporting method development.

February 6, 2024 GMT