Using a Single Quadrupole Mass Spectrometer to Check Peptide Synthesis and Analyze Impurities

User Benefits

- A single quadrupole mass spectrometer can be used in combination with an HPLC system to check for known compounds or quickly predict the molecular weight of unknown impurities. - Designed for easy maintenance, the LCMS-2050 high performance liquid chromatograph mass spectrometer helps shorten instrument downtimes and reduce the time and labor required for maintenance.

Introduction

When analyzing peptides, mobile phases containing trifluoroacetic acid (TFA) are commonly used for analyzing purity levels by HPLC. This article describes an example of measuring a commercial peptide standard sample to check molecular weights and analyze impurities by MS using TFA- based mobile phase conditions and optional deconvolution software. The LCMS-2050 is a compact single quadrupole mass spectrometer, which was developed specifically for easy operability and is a powerful tool for easily obtaining mass information that uses the same operating methods as HPLC systems. The LCMS-2050 is also designed for easy maintenance, which helps reduce instrument downtime and the time and labor required for maintenance.

20 de febrero de 2024 GMT