Multi-omics Analysis Using Next-Generation Sequencer and Mass Spectrometer in Longevity Research

Introduction

Using a next-generation sequencer (GridION, Oxford Nanopore Technologies) and a liquid chromatograph-mass spectrometer system (LCMS-9050 with Nexera Mikros™), we compared long-lived Drosophila melanogaster with its wild type. In DNA analysis, 830,000 SNVs/Indels (approximately 40,000 structural variants) were detected by whole genome sequencing, and in RNA analysis, 185 loci with a p-value of 0.05 or less were detected by comprehensive expression analysis. About 1,000 proteins were identified by comprehensive protein analysis, and about 300 metabolites were detected by wide-target analysis. These results were integrated into the Multi-omics Analysis Package to study longevity mechanisms.

April 22, 2024 GMT