Analysis of Compositional Protein Behavior by Bacterial Culture Time

User Benefits

- Multivariate analysis tools in eMSTATSolution can reveal differences that cannot be determined by visual appearance or bacterial counts. - The annotation function can be used to estimate the composition proteins contained in a sample. - MALDI-8020/30 is fast and can analyze large amounts of samples in a short time.

Introduction

Differences in culture conditions for microorganisms, such as medium, temperature, and time, can change what is detected when microorganisms are analyzed. This is because environmental stress and the cultivation phase cause changes in the products in the organism. Therefore, when analyzing and identifying microorganisms, it is necessary to optimize the culture conditions and pretreatment methods under which the target substance is produced using standard microorganisms before culturing and analyzing unknown microorganisms. These conditions require consideration of multiple factors, and the number of samples to be analyzed is often large. In this application news, as an example of the examination of the conditions for the bacterial body analysis, Escherichia coli strain DH5α, which is used as a calibration sample for the identification of microorganisms, was cultured, and changes in the composition proteins detected over time were analyzed using MALDI-8030 and eMSTAT Solution.

August 27, 2024 GMT