Metabolites Analysis in Mouse Brain Using the Image Analysis Function of IMAGEREVEAL MS

Introduction

In recent years, matrix-assisted laser desorption/ ionization-mass spectrometry imaging (MALDI-MSI) has improved instrumental performance, enabling the acquisition of a large number of peaks at a time. Until the mid-2000s, those obtained peaks were manually analyzed since MALDI-MSI was first reported in 1997. These days, however, various analysis methods that utilize statistical analysis techniques have been proposed. In this application note, MALDI-MSI was performed using iMScope QT equipped with a quadrupole time-of-flight mass spectrometer, and IMAGEREVEAL MS, a dedicated MSI software, was used for the analysis. iMScope QT can acquire data with high mass resolution and high mass accuracy. IMAGEREVEAL MS also allows even inexperienced users to perform statistical analysis of the obtained data easily and quickly. This application note describes one method for efficiently analyzing signals obtained from mouse brain sections using iMScope QT in combination with IMAGEREVEAL MS.

25 de septiembre de 2024 GMT