Simple Labor-Saving Calibration Curve Creation Using Autosampler Automatic Dilution Function part 2

User Benefits

- The autosampler’s automatic dilution function reduces manual dilution preparation and organic solvent consumption. - Simply specify the desired dilution ratio in the batch table, and use the same method file to automatically dilute solutions and create a calibration curve. - Setting and management are easy when changing HPLC conditions since a single method file is used regardless of the dilution ratio.

Introduction

The dilution of standard and sample solutions for HPLC analysis is generally performed manually, using pipettes. However, such work is labor-intensive and time-consuming. In recent years, automation for the purpose of labor-saving is desired to improve work efficiency and productivity. When the organic solvent is a diluent, a large amount of solvent is consumed to prepare standard solutions for calibration curves in volumetric flasks, but the volume of sample solution required for HPLC analysis is only a few tens of μL or less. Using the automatic dilution function equipped with Nexera Autosamplers, it is possible to prepare a sample diluted at a user-defined factor and introduce it directly into the analytical column. The Application News 01-00717 describes a simple method for creating calibration curves using ultrapure water as a diluent. This article introduces the analysis using organic solvent as a diluent.

26 de noviembre de 2024 GMT