Analysis of Phenethylamines Using On-Column TFA Derivatization

User Benefits

- Trifluoroacetyl (TFA) derivatization increases the ability to differentiate between phenethylamines which otherwise provide poor mass spectral information. - Automatic derivatization shortens sample pretreatment time. - Two-step injection is now a standard function of LabSolutions GCMS and is available through software updates.

Introduction

Phenethylamines, such as methamphetamine and MDMA, are illegal drugs that are abused worldwide. Phenethylamines are prone to adsorption due to their amine backbone, and analysis of the unchanged phenethylamines provides poor mass spectral information. Therefore, they are commonly derivatized to make them more easily differentiated. Trifluoroacetic (TFA) anhydride is typically used for the analysis of phenethylamines. However, conventional derivatization by anhydrous TFA is time-consuming as it requires drying of the derivatization reagent after derivatization. This Application News presents the analysis of phenethylamines by automatic on-column derivatization using auto-injector AOC-30i and a LabSolutions GCMS system that is now equipped with 2-step injection as a standard function. On-column derivatization allows for the direct analysis of extracted urine samples without additional sample pretreatment, which can significantly shorten the time required for derivatization.

5 de diciembre de 2024 GMT