Short-Chain Oligonucleotide Analysis Using Supercritical Fluid Chromatography

Introduction

The applicability of supercritical fluid chromatography (SFC) to the analysis of impurities in oligonucleotide was evaluated. In this study, we demonstrated that 4-mer oligonucleotides can be analyzed by optimizing the analytical conditions. As a result of comparative studies, 4-mer oligonucleotides with different amounts of phosphorothioate modification could be separated using a mixture of methanol and water containing 2-aminoethanolacetic acid as a modifier (co-solvent) and Shim-pack^TM UC-Diol ll as a column. These results demonstrate the applicability of this method to oligonucleotide analysis.

17 de diciembre de 2024 GMT