Molecular Weight Measurement and Sequence Confirmation- Oligonucleotide Therapeutics

This single quadrupole mass spectrometer can be integrated into an LC system and operated with the same ease as an LC detector. Due to its wide mass range from m/z 2 to 2000 and the large number of detectable valence ions, it is possible to calculate the molecular weight of oligonucleic acids and other medium-sized molecules with high reliability using the deconvolution function. When analyzing using a UV detector in combination with the LCMS-2050, it is also possible to overlay mass information on the chromatograms.

The LCMS-TQ RZ series triple quadrupole mass spectrometers are designed to offer the three “Rs” of reliability, resilience, and responsibility. The series offers improved safety and provides worry-free analytical solutions to ensure that highly reliable data can be acquired.

This quadrupole time-of-flight (Q-TOF) mass spectrometer offers the highest class of mass accuracy reliability. Shimadzu’s unique high-precision temperature control system suppresses even tiny mass variations caused by external factors, ensuring accurate mass measurements without the operator worrying about mass calibration. Insight Biologics oligonucleic acid sequencing software can identify oligonucleic acid impurities and confirm sequences with predicted modification positions.

Operations to identify oligonucleic acid impurities or to predict the position of modifications based on complex mass spectra are typically extremely time-consuming. Moreover, using multiple software programs for measuring masses and analyzing data makes operations complex and increases the operator’s workload. Insight Biologics solves such problems and provides a far better user experience.

The body of the bio-inert HPLC column is coated with a newly developed bioinert coating, and the column is packed with an organic silica hybrid stationary phase. Highly reproducible, high-sensitivity nucleic acid analysis results are obtained from the first injection. Another feature is high durability.

This compact tabletop MALDI-MS system enables quick and easy oligonucleic acid analysis. Mass measurements are possible without sample fragmentation. Furthermore, MALDI-ISD (in-source decay) enables simple and clear sequence analysis by acquiring mass spectra based on simple cleavage.

This compact tabletop MALDI-MS system enables quick and easy oligonucleic acid analysis. Mass measurements are possible without sample fragmentation. Furthermore, MALDI-ISD (in-source decay) enables simple and clear sequence analysis by acquiring mass spectra based on simple cleavage.