Improved Analytical Workflow for Phospholipids by Nexera-e and Co-Sense for Impurities : SHIMADZU (Shimadzu Corporation)

Together, the comprehensive two-dimensional Nexera-e, offering exhaustive analysis of diverse compounds, and the Co-sense series, permitting high resolution and enrichment of specific fractions, make it possible to compare the overall compound patterns in the target sample. Combination of the Nexera-e and Co-Sense series also allows precise separation of the elution fraction of interest.
With this method, the two workflows consisting of the differential analysis of a sample with a complex matrix, and a detailed analysis of the compounds of those results to reveal differences, can be conducted simultaneously, quickly and conveniently. Here, we introduce an actual example using phospholipids.

Table 1 Analytical Conditions for 1D Separation of Phospholipids

Fig. 1 1D Reversed Phase Separation of Phospholipids

Fig. 2 2D Plot of All Positive MRM Transitions of Phospholipids

Nexera-e

Features:

The comprehensive 2D-LC methodology is a paradigm shift in liquid chromatography separation analysis. By combining two independent separation modes orthogonally in combination with a dual-loop/dual-valve alternate switching design, the highest possible peak capacity is achieved for LC chromatography separation.

Co-Sense Series

Features:

The Co-Sense series features automatic sample processing systems intended to improve the efficiency of LC-NMR and LC-MS operations by automating complicated sample processing steps. This eliminates the need for tedious, time-consuming, and expensive manual processes.