LC/MS/MS Method Package for Glycosaminoglycan

The study of Glycosaminoglycans (GAGs) or mucopolysaccharides, and enzymes involved in GAG metabolism is an important aspect of clinical research related to lysosomal storage. Quantitation of GAGs in bones, joints, the brain, heart, lungs, and other organs is a useful tool in this field of study. GAGs can be quantitated by measuring the sulfated disaccharides produced by enzymatic degradation of GAGs. The research group led by Hironori Kobayashi of Shimane University Hospital, Clinical Laboratory Division, has been instrumental in the development of methods for the detection of sulfated disaccharides by LC/MS.

The LC/MS/MS Method Package for Glycosaminoglycans contains LC/MS/MS analytical methods with optimized separation conditions and MS parameters for sulfated disaccharides derived from six important glycosaminoglycans. It also has example sample preparation protocols for blood spots, including enzymatic preparation. This method package can be used to analyze glycosaminoglycans while saving significant time in the development of sample preparation and analytical protocols.