Shim-pack Velox C18
HPLC Column
C18
Shim-pack Velox C18 is a traditional end-capped C18-bonded phase which offers the highest hydrophobic retention of any Shim-pack Velox phases and is applicable to a wide range of applications such as pharmaceutical, food, environmental and clinical and neutrals at moderately low and mid-range pH.
- General purpose column for reversed-phase chromatography
- Highest hydrophobic retention among Shim-pack Velox series
- Compatible with moderately acidic to neutral mobile phases (pH 2-8)
Method Transfer for Cyanocobalamin Analysis within the USP Allowable Adjustment
The assay of cyanocobalamin (a synthetic form of vitamin B12) with 5 μm fully-porous ODS column described in the USP monograph is transferred to a new method with Shim-pack Velox C18 2.7 μm column, within USP allowable adjustments. Analytical time and solvent consumption can be saved with transferred methods while meeting the requirements of system suitability.
| Column | L/dp | Flow rate (mL/min) |
N | System suitability test result (Requirement: %RSD < 2.0 %) |
|---|---|---|---|---|
| VP-ODS (5 μm, 4.6 x 150 mm) |
30,000 | 0.50 | 5,244 | tR: 0.025 % Area: 0.175 % (n=6) |
|
Velox C18
(2.7 μm, 4.6 x 100 mm) |
37,037
(+23 %) |
0.50 | 9,497 (+81 %) |
tR: 0.035 % Area: 0.103 % (n=6) |
| 0.93 | 4,466 (-15 %) |
tR: 0.084 % Area: 0.220 % (n=6) |
Applications
| Documents | Date Creation Date |
|---|---|
2022-11-24 | |
2021-12-27 | |
2021-12-13 |
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