Shim-pack SCR-101N/C/P are suitable for the analysis of monosaccharides. Since the samples are separated under a mixed mode of gel filtration and ligand exchange, the selectivity differs depending on the type of cation.
Reducing Sugar Analysis System - Columns
Nexera Reducing Sugar Analysis System
Borate Complex-Anion Exchange
The Shim-pack ISA-09 column uses the property of sugar to form a complex with boric acid and separates this in anion exchange mode. Monosaccharides and disaccharides are separated simultaneously with a gradient in which boric acid buffer solutions at two different concentrations are used as the mobile phase.
| Column | Particle Size (µm) | Size (I.D. × L mm) | P/N |
|---|---|---|---|
| Shim-pack ISA-09 | 9 | 4.0 × 250 | S228-59512-41 |
| Shim-pack ISA-09 (G) | － | 4.0 × 50 | S228-59512-42 |
Gel filtration-Ligand Exchange Column
The Shim-pack SCR series columns are for ligand exchange chromatography and use the property of some metallic ions to form complexes with sugars. Water is used as the mobile phase, but anomer separation can be seen when running the analysis at low temperatures.High tepemrature (60 °C or higher ) is recommended to avoid this phenomenon.
| Column | Particle Size (µm) | Size (I.D. × L mm) | P/N |
|---|---|---|---|
| Shim-pack SCR-101N | 10 | 7.9 × 300 | S228-07730-92 |
| Shim-pack SCR-101C | 10 | 7.9 × 300 | S228-17889-91 |
| Shim-pack SCR-101P | 10 | 7.9 × 300 | S228-17890-91 |
| HPLC column, guard column SCR (N) | － | 4.0 × 50 | S228-09619-92 |
| HPLC column, guard column SCR (C) | － | 4.0 × 50 | S228-17891-91 |
| HPLC column, guard column SCR (P) | － | 4.0 × 50 | S228-17892-91 |