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    Benchtop MALDI-TOF Imaging Starter Kit - Applications

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    Analysis of lipids in full rat brain with 30 µm and 50 µm spacing

    Analysis of lipids in full rat brain with 30 µm and 50 µm spacing

    MALDI images of full rat brain using FlexiVision-mini ITO glass slide. A, optical scan; B, positive ion mode image from MALDI-8020 using 50 µm spacing; C, negative ion mode image from MALDI-8030 using 30 µm spacing.

    Sample B

     
    Matrix DHB, sublimated with Shimadzu iMLayer device
    Measurement region 67,024 pixels
    Measurement time around 1.9 hours
    Experiment details Laser repetition rate of 200 Hz and 20 shots per pixel

    Sample C

     
    Matrix 9-AA, sublimated with Shimadzu iMLayer device
    Measurement region 275,210 pixels
    Measurement time around 3.8 hours
    Experiment details Laser repetition rate of 200 Hz and 10 shots per pixel

    Large molecule imaging (protein/ on-tissue digestion) with 50 µm spacing

    Large molecule imaging

    MALDI images of protein and peptides in full rat brain using FlexiVision-mini ITO glass slide and MALDI-8020. A, MBP protein, (m/z 14124); B, MBP peptide (HGFLPR, m/z 726.39)

    Sample A

     
    Matrix Sinapinic acid, sprayed with Shimadzu iMLayer AERO device
    Measurement region 68,836 pixels
    Measurement time around 3.8 hours
    Experiment details Laser repetition rate of 100 Hz and 20 shots per pixel

    Sample B

     
    Matrix CHCA, sprayed with Shimadzu iMLayer AERO device
    Measurement region 67,482 pixels
    Measurement time around 2.8 hours
    Experiment details Laser repetition rate of 200 Hz and 30 shots per pixel