MRM Based Free Fatty Acids Profiling in Human Plasma and Serum Using LC-MS/MS

User Benefits

- Simultaneous analysis of 19 free fatty acids in blood was allowed with simple pretreatment.
- The delay column worked to separate the target fatty acids from the undesirable contaminants.
- LCMS-8060RX newly equipped with CoreSprayenables stable and reliable quantitative analysis for trace amount of compounds.

Introduction

Free fatty acids, about 5% of the total fatty acids in the body, are known to be important signal transducers that regulate functions such as insulin secretion. It is also attracting attention as a biomarker for various endocrine disorders.
Gas chromatography (GC) or gas chromatography-mass spectrometry (GC/MS) generally requires pretreatment such as methyl esterification for long-chain fatty acids and polyunsaturated fatty acids analysis. Multiple reaction monitoring (MRM) using liquid chromatography-mass spectrometry (LC/MS) enables highly sensitive quantitative analysis of fatty acids without derivatization. However, fatty acids widely found in the environment, such as palmitic acid and stearic acid, are introduced as impurities in LC system.
This report presents an example of quantitative analysis of free fatty acids in human plasma and serum using the highly stable LCMS-8060RX system coupled with a delay column.

October 8, 2024 GMT