Technology for enabling use wide flow rates range from micro to semi-micro
When using LC-Mikros, the valves can be switched into three different states: only pump A is connected to the mixer, only pump B is connected to the mixer, or both pump A and B are connected to the mixer.
As a result, the back flow of the solvent occurred when either pump A or B is off is reliably prevented, and the micro flow rate is achieved.
The LC-Mikros solvent delivery pump has completely new direct-drive engineering to deliver a wide range of flows from micro to semi-micro(1-500 μL/min) in both isoclatic and gradient elution modes.
Thus, a simultaneous analysis of multicomponent ranging from polar to hydrophobic compounds can be realized.
Flowrate: 5 μL/min
Column: Shim-pack MC C18 (0.3 mmID x 150 mm)
*Obtainded under our specified conditions.
Column: Shim-pack MC C18 (0.3 mmID x 150 mm)
*Obtainded under our specified conditions.
| Average Retention Time | Standard Deviation | RSD | |
|---|---|---|---|
| Gly-Tyr | 5.31 | 0.009 | 0.16% |
| Val-Tyr-Val | 7.97 | 0.006 | 0.07% |
| Angiotensin II | 9.32 | 0.015 | 0.16% |
| Leu enkephalin | 9.71 | 0.011 | 0.11% |
| Met enkephalin | 10.5 | 0.012 | 0.11% |
| Time (min) | 1 | 11 | 11.1 | 13 | 13.1 | 25 |
|---|---|---|---|---|---|---|
| Concentration B | 1% | 40% | 90% | 90% | 1% | 1% |
