Enumeration and Morphological Evaluation of Spheroids (Cell Clusters) by iSpect DIA-10

User Benefits

- It is possible to quickly detect almost all particles in the sample solution (for a 100 μL sample solution, it takes a few minutes). - With the autofocus function that does not require a standard sample, focusing can be completed in about 15 seconds. - By filtering based on shape parameters, it is possible to count spheroids only, even in the presence of single cells mixed in the sample.

Introduction

Spheroids (cell clusters) are cellular aggregates produced by a three-dimensional culture, unlike a conventional two- dimensional culture. Cell clusters such as spheroids exhibit physiological functions closer to those of living organisms than adherent cells produced by two-dimensional cultures, making them useful for cell therapy and drug sensitivity testing. Understanding the concentration and size of spheroids is important for investigation of their culture conditions, quality control, and research applications. The Shimadzu iSpect DIA-10 dynamic particle image analysis system, which is based on the dynamic image analysis method, is an instrument that acquires images of particles in liquid samples and measures the particle size distribution, concentration, and shape. With an optical system that misses very few particles and has an image acquisition efficiency of 90% or higher, it is possible to analyze tens of thousands of particles and extract and count spheroids by setting filtering conditions. This article introduces an example in which suspensions of HEK293 single cells and spheroids were evaluated using the iSpect DIA-10.

June 29, 2023 GMT