Pre-column Amino Acid Analysis of Hydrolyzed Alternative Proteins

User Benefits

- Very easy amino acid analysis can be performed because of automated derivatization involving complicated processes. - Samples treated through three different hydrolyses (hydrochloric acid hydrolysis, alkaline hydrolysis, and performic acid oxidation) can be analyzed using a same HPLC instrument. - Two different analytical methods can be employed using the same column, the same reaction reagents.

Introduction

Hydrochloric acid hydrolysis is commonly used as a pretreatment when analyzing amino acids constituting proteins and peptides. During hydrochloric acid hydrolysis, asparagine and glutamine are converted to aspartic acid and glutamic acid, respectively. Tryptophan and the sulfur-containing amino acids methionine, cysteine, and cystine are decomposed in the process, making accurate determination impossible. To achieve accurate determination, alkaline hydrolysis is used in the determination of tryptophan. For methionine, cysteine, and cystine, performic acid treatment oxidizes methionine to methionine sulfone and cysteine and cystine to cysteic acid. Hydrochloric acid hydrolysis is then used to determine methionine sulfone and cysteic acid, respectively. In this article, Analysis of alternative proteins hydrolyzed by a pre-column derivatization method using Nexera XR, high performance liquid chromatograph, is presented.

January 28, 2025 GMT