Determination of Various PFAS in Egg Matrix Using Stacked Injection On-line SPE Coupled to LC-MS/MS

User Benefits

- Single vendor solution for UHPLC and MS system - Quantification of 27 PFAS in ng/mL range using an on-line SPE approach - Increased sensitivity due to the stacked injection combined with on-line SPE

Introduction

Perfluoroalkyl and polyfluoroalkyl substances (PFAS) refer to a class of more than 4000 individual chemicals that have been widely used since the 1950s, e.g. as fire retardants, food packaging materials or non-stick coatings. These compounds offer heat-resistant, and oil- and waterrepellant properties as well as chemical and thermal stability, resistance to UV light and weathering. Due to their anthropogenic origin, PFAS cannot be degraded, and hence they accumulate and can now be detected ubiquitously in the environment. Due to this PFAS also found their way into the food chain and accordingly into our food. Concerns about human exposure through diet, studies on the status of food contamination are being conducted in various countries. Here we describe the determination of various PFAS in egg matrix in a relevant concentration range. The analysis is based on a simple QuEChERS extraction coupled to an online SPE approach. This omits additional sample preparation steps like dSPE.

February 11, 2025 GMT