Efficient Optimization of Separation Conditions for Synthetic Peptide Using Super Critical Fluid Chromatography

User Benefits

- Supercritical fluid chromatography (SFC) can be applied to hydrophilic peptide analysis. - Efficient workflow of optimizing separation conditions using SFC can be provided by LabSolutions MD. - The mobile phase blending function enables automated preparation of mobile phases, contributing to the improvement of efficiency and reproducibility.

Introduction

Supercritical fluid chromatography (SFC) has advantage of shorter analysis time than liquid chromatography (HPLC) due to the large diffusion coefficient and low viscosity of carbon dioxide used as the mobile phase, and it is superior in separating structurally similar substances. SFC has been developed as an analytical method for chiral compounds, but in recent years its application has expanded to separation and analysis of achiral compounds.SFC is excellent for the separation and analysis of hydrophobic compounds based on the hydrophobic nature of carbon dioxide, but by adding a highly polar organic solvent (modifier) such as methanol to the mobile phase, SFC can also be used for the analysis of hydrophilic compounds. Peptides, a typical hydrophilic compound, are generally analyzed using reversed-phase HPLC, but SFC is also available; since SFC shows different retention behavior from LC, it can be expected to provide satisfactory separation that cannot be done using LC. In this article, Automated workflows in screening” and “optimization” phases using a standard peptides mixture as a simulated sample, using the supercritical fluid chromatograph Nexera UC and the analytical method development support software LabSolutions MD.

February 13, 2025 GMT