HPLC Analyses of Nucleotides in Powdered Infant Formula and Liquid Infant Formula

User Benefits

- Five nucleotides in powdered infant formula and liquid infant formula can be simultaneously analyzed by HPLC. - Nucleotides can be determined precisely owing to the optimization of pretreatment procedure and analytical method to reduce the influence of foreign substances with reference to ISO and AOAC official methods.

Introduction

Powdered infant formula and liquid infant formula are essential for providing optimal nutrition to infants. They are manufactured to make their composition close to those of breast milk. Nucleotides in breast milk have not been fully characterized in comparison with other key nutrients such as proteins and fats. However, important physiological functions of nucleotides have been revealed in recent years. For example, nucleotides have been reported to improve intestinal development, digestion/absorption, and allergy-prevention of an infant. Furthermore, nucleotides play an important role in the development of lipid metabolism and brain function and are now included in infant formulae as a semi-essential nutrient. In this application news, analyses of five nucleotides in powdered infant formula and liquid infant formula using the Nexera XR high performance liquid chromatograph is introduced. The pretreatment method and HPLC analytical conditions are based on ISO 20638:2015(E) published by the International Organization for Standardization (ISO) and the AOAC Official Method 2011.20 published by AOAC INTERNATIONAL, a North American organization that standardizes food testing methods and validates analytical methods.

March 18, 2025 GMT