Simultaneous Analysis of Synthetic Dyes Including Red No. 3 Using Organic Silica Hybrid Core-Shell Columns

User Benefits

- The organic silica hybrid-based Shim-pack NovaCore C18-HB provides a wide pH tolerance range from 1 to 12 for both the mobile phase and sample solvent. - A reduction in analysis time compared to fully porous columns can be achieved because separation on the Shim-pack NovaCore C18-HB column occurs only in the porous outer layer of the particle (core-shell column). - LabSolutions MD, dedicated software for supporting method development, enables efficient exploration of separation conditions.

Introduction

Food colorants can be categorized into natural and synthetic types. The availability of synthetic colorants varies across countries, depending on regulatory frameworks. In Japan, twelve synthetic colorants are currently approved as food additives, and their safety can be assessed through qualitative and quantitative analysis using liquid chromatography. Recently, the U.S. Food and Drug Administration announced a prohibition on the use of Red No. 3, one of these twelve colorants, in food products, further increasing interests regarding the safety of synthetic colorants. This study presents the optimization of separation conditions for the simultaneous analysis of these twelve synthetic colorants using the organic silica hybrid core-shell column, Shim-pack NovaCore C18-HB, in combination with LabSolutions MD, a dedicated software for supporting method development. Furthermore, the applicability of the optimized method is demonstrated through the analysis of a real sample, Konpeito sugar candy.

March 31, 2025 GMT