UV-Simple Quantitative Measurement of Nannochloropsis Micro Algae in Water Using Shimadzu UV Micro Algae Analysis System

We introduce Shimadzu's newly developed UV micro algae analysis system which permits simple measurement of the micro algae concentration using a spectrophotometer. Measurement of a single sample can be accomplished very quickly in about one minute using a disposable screw-cap vial (or disposable cell). 

• Measurement of a single sample can be accomplished very quickly in about one minute using a disposable screw-cap vial (or disposable cell)
• The use of disposable screw-cap vials for these measurements eliminated the need for manual operations such as the washing of vials, which also eliminated any concerns regarding contamination and carry-over due to insufficient washing.

   

  

   

  Uv-2600i

   

Concentration and Plot of reflectance at 910 nm
 

 

Actual Concentration (%)	Predicted Results by Single Regression Method (%)(*)	Predicted Results by Multiple Linear Regression Method (%) (**)
70	72.9	69.2
70	73.4	69.1
30	32.4	28.3
30	32.3	30.0
5	1.4	5.3
5	1.5	5.4

Using samples other than 70, 30 and 500 million cell/ml as standard samples, calibration curves of the single regression method (Primary calibration curve: Figure 3) and the multiple linear regression method were prepared, and the concentrations of samples of 70, 30 and 500 million cell/ml were quantitatively calculated. As shown in Table 1, the single regression method gives good predicted results, but multiple regression gives more accurate predicted results.

• The cell mass is large and the reflectance is high, a reflection method using a disposable sample bottle
• The cell mass is small and the reflectance is low,  a transmission method using a disposable cell.