The synthesis and purification processes for oligonucleotide therapeutics generate many types of impurities. New analytical methods continue to be developed to analyze such impurities in more detail.

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Nexera UC
Supercritical Fluid Extraction / Supercritical Fluid Chromatograph

The properties of supercritical carbon dioxide, the main mobile phase used for supercritical fluid chromatography (SFC), include 1) polarity nearly as low as hexane and 2) lower viscosity and higher diffusivity compared to liquid. These properties can be expected to generate separation selectivity that differs from conventional HPLC. In addition to enabling high-separation analysis, the properties also enable simultaneous analysis of components with a wide range of polarities, increase the sensitivity of mass spectrometers, and offer other analytical capabilities not available with conventional HPLC.