iMLayer AERO - Features
Automatic Sprayer for MALDI Imaging
Imaging with High Reproducibility
Humidity Control
Spraying can be performed under more stable conditions than with a hand spray because the atmosphere within the spray chamber is replaced before pretreatment.
Clog-Free Reagent Delivery
If the matrix clogs the nozzle tip, the spray becomes unstable, which can lead to lower reproducibility. The rinsing mechanism allows for clog-free, stable spraying, which enables high reproducibility in MALDI analyses.
Both High Sensitivity and Spatial Resolution Achieved
Excellent Spray Performance
The standard protocol provides sensitivity on par with a hand spray along with improved resolution, homogeneity and reproducibility
Two-Step Vapor Deposition
To acquire high spatial resolution (5 to 10 µm) images and increase sensitivity, a two-step vapor deposition method can be implemented in combination with the iMLayer matrix vapor deposition system.* This unique experiment can only be implemented using Shimadzu sample preparation solutions.
* Patents: JP6153139 and JP6183779
Two-Step Vapor Deposition Allows for Very Fine Images with Minimal Blur
Hand Spray
- Matrix：9-AA
- Volume used：200 μL
Two-Step Vapor Deposition
- Film thickness：1 μm
- Solution delivery volume：120 μL/min
- Stage speed：70 mm/sec
- Laminating layers：4
Applicable to Pretreatment Other than Matrix Coatings
Derivatization Treatment
The system is also compatible with derivatization treatment (Girard's reagent T).
A single iMLayer AERO unit provides extensive MSI pretreatment coverage.
Using derivatization can improve the ionization efficiency of testosterone and other substances that are difficult to ionize.