LC/MS/MS Method Package for Reactive Sulfur Profiling

Sample Pretreatment Protocols and Analytical Methods for 17 Sulfur-Containing Metabolites,Including Reactive Sulfur Species

Oxidative stress and the subsequent degradation of biological molecules related to various reactive oxygen species is an important field of clinical research. Recently, an expansive sulfur metabolome investigation conducted by Professor Takaaki Akaike et al. of Tohoku University Graduate School of Medicine has shown that cysteine persulfide and other reactive sulfur species play an important role in biological defense and antioxidant response systems. Reactive sulfur species are found in every organ of the body, including the brain, heart, and liver, and are even found in the blood in healthy people. Researchers are investigating the role of these compounds in relation to aging and conditions associated with oxidative stress such as respiratory disease, heart disease, and cancer.
This LC/MS/MS Method Package for Reactive Sulfur Profiling offers analytical methods for LC/MS/MS that target all 17 sulfur-containing metabolites, including reactive sulfur species, with separation conditions and MS parameters that are optimized for each compound. It also comes with example pretreatment protocols, including derivatization steps, for biological samples containing cells and blood plasma. The method package allows the user to analyze sulfur-containing metabolites without time-consuming investigation into sample preparation and analytical conditions. The Shimadzu Multi-omics Analysis Package also offers a tool to visualize quantitative results obtained with this method package. The tool helps users interpret results by providing easy-to-understand graphical displays of the relationships and ratios between metabolic components in different sample sets.