Shim-pack GIS Series - Features

HPLC Column Solutions

Column Chemistries

  Shim-pack
GIS C18
Shim-pack
GIS C18-P
Shim-pack
GIS RP-Shield
Shim-pack
GIS C8-L
Shim-pack
GIS CN
Shim-pack
GIS SIL
Shim-pack
GIS HILIC
Bonded Phase Octadecyl Groups Octadecyl Groups Octadecyl Groups Octyl Group Cyanopropryl Group - Diol Group
Analysis Mode Reversed Phase Reversed Phase Reversed Phase Reversed Phase Reversed Phase, Normal Phase Reversed Phase, Normal Phase​ HILIC
Features High retentivity, lower columnback pressure, high inertness High Steric Selectivity Embedded with a Polar Functional Group Weak hydrophobic interactions, High inertness Suitable in either reversedphase or normal phase mode Suitable in either reversed phase or normal phase mode​ Ideal for the Separation of Highly Polar Basic Compounds
Particle Size (μm) 2, 3, 4, 5, 10 3, 5 5 3, 5 3, 5 3, 5 3, 5
Pore Size (nm) 10 10 10 10 10 10 10
Surface Area (m2/g)
450 450 450 450 450 450 450
Carbon Loading (%) 15 29 9 5 14 - 20
End Cap - - - - -
pH Range 2-7.5 2-7.5 2-7.5 2-7.5 2-7.5 2-7.5 2-7.5
USP Code L1 L1 L1 L7 L10 L3 L20

High retentivity, lower column back pressure, high inertness

Analysis Examples

Food Dyes

Peaks

 1. Tartrazine  7.6 mg/L
 2. Amaranth  3.8 mg/L
 3. Ingigocarmine  7.6 mg/L
 4. New coccine  3.8 mg/L
 5. Sunset Yellow FCF  5.3 mg/L
 6. Naphthol Yellow S  7.6 mg/L
 7. Uranine  3.8 mg/L
 8. Allura Red AC  5.3 mg/L
 9. Ponceau R  7.6 mg/L
 10. Ponceau SX  5.3 mg/L
 11. Orange I  5.3 mg/L
 12. Fast green FCF  3.0 mg/L
 13. Brilliant blue FCF  3.0 mg/L
 14. Ponceau 3R  7.6 mg/L
 15. Erythrosine  5.3 mg/L
 16. Azure Blue VX  3.0 mg/L
 17. Orange II  7.6 mg/L
 18. Acid red  3.0 mg/L

 

Conditions

 Column  Shim-pack GIS C18 (150 mmL. x 4.6 mmI.D., 4 μm)
 Eluent  A) 10mM Na2HPO4 (pH6.9 ; 10mM NaH2PO4)
 B) CH3CN
 A/B = 90/10 - 50 min - 65/35
 Flow Rate  1.0 mL/min
 Detection  UV 270 nm
 Col. Temp.  40 °C
 Injection Vol.  10 μL

Free Amino acids and Organic acids

Conditions

 Column  Shim-pack GIS CN
 (250 mmL. x 4.6 mmI.D., 5 μm)
 Eluent  20 mM KH2PO4 (pH 4.0; H3PO4)
 Flow Rate  1.0 mL/min
 Col. Temp.  40 °C
 Detection  UV 210 nm
 Injection Vol.  5 μL
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