Study on the Mechanism of Early Delivery by Multi-Omics Analysis of Metabolites, Elements and Bacterial Flora in Amniotic Fluid

Introduction

A gas chromatograph mass spectrometer (GCMS-TQ™8040 NX) and an energy-dispersive X-ray fluorescence spectrometer (EDX-7200) were used to analyze metabolites (488 components) and elements (78 elements) contained in the amniotic fluid of pregnant women. These data were combined with amniotic fluid microbiota (approximately 250 species of microorganisms) measured using a miniature next-generation sequencer (MinION Mk1C System, Oxford Nanopore Technologies, Inc.) and 16 items of labor data (e.g., neonatal weight) to analyze differences between preterm and normal births. Volcano plot and metabolic pathway analyses were performed using Multi-omics Analysis Package based on the 740 items detected. In this application, we introduce an example of multi-omics analysis conducted by gender and time series.

September 9, 2024 GMT