TECHNICAL PAPERS
Automated Microchip Electrophoresis System
Shimadzu Review 74[3・4] (2017.12)
Abstract
High-throughput quantitative and qualitative glycoanalytical procedures are required for the glycoscientific research field (e.g. metabolism of glycans, glyco-biomarker discovery, and quality control of glycoprotein pharmaceuticals). Among currently available analytical techniques, microchip electrophoresis is one candidate for high-throughput quantitative and qualitative glycoanalysis. In this article, we describe a strategy for high-throughput glycoanalytical procedures for the relative quantitation of N-linked glycans using the MultiNA microchip electrophoresis system, which is specialized for DNA/RNA analysis. We designed suitable derivatization of glycans for fluorescence detection in MultiNA and optimized separation conditions for derivatized glycans without any hardware modification. APTS-labeled N-glycans released from some glycoproteins were successfully separated based on the difference in branching type and terminal modifications within 1.5 min. Furthermore, we also designed microchip lectin-affinity electrophoresis for glyco-profiling. Thus, we showed that microchip electrophoresis provides high-throughput quantitative and qualitative glyco-analytical procedures and expect that MultiNA will provide a new dimension to glycoscience in the field of glycobiology.
Keywords: Microchip electrophoresis, Glycoprotein, N-glycans, Glycoanalysis
1Faculty of Pharmacy, Kindai University, Osaka, Japan
2Global Application Development Center, Analytical & Measuring Instruments Division, Shimadzu Corporation, Kyoto, Japan
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