Shimadzu Review Vol.80[3・4](2023)
Diagnostics

SPECIALLY COLLECTED PAPERS

Study of Direct Bacterial Identification and Susceptibility Testing of Positive Blood Culture Samples

by Hiroshi Iwata

Shimadzu Review 80[3・4] (2023)

Abstract

The early administration of effective antibiotics is essential for the treatment of bloodstream infections. However, current bacterial testing requires several days to return the bacterial identification and antimicrobial susceptibility results needed to select an effective antibiotic. This is a lengthy process that demands to be streamlined. In this article, we present the results of using standard bacterial strains in a short sample treatment step that replaces the overnight secondary culture normally performed after blood culture. Bacterial identification by MALDI-TOF MS and susceptibil-ity testing were performed directly on blood culture samples treated by this method without an overnight secondary plate culture. Using MALDI-TOF MS, the bacteria in all samples were identified to the genus level or above. In addi-tion, good susceptibility test results were achieved with an essential agreement of 94.9% and categorical agreement of 97.8% compared with the conventional secondary plate culture method.


Clinical and Microbiological Testing Business Unit, Diagnostics Management Department, Analytical & Measuring Instruments Division, Shimadzu Corporation, Kyoto, Japan

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