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Shimadzu Review 80[3・4] (2023)
Abstract
Luciferases are reporter proteins with advantages such as a wide dynamic range, high detection sensitivity, low phototoxicity, and ease of use to observe deep region in vivo. Advantages of small luciferase include a high possibility to express correct protein structures when fused with a target or target-binding protein, a low risk to cause steric hindrances with respect to functions of target or target-binding proteins, easier genome insertion due to the short gene that encodes the luciferase, and suitability for BRET-based detection systems.
This article describes the smallest luciferase (picALucTM) developed with a practical brightness. In addition to the small size and brightness of picALuc, they also offer high thermal stability and can be produced in large quantities. That makes them especially useful as reporter proteins.
Biology･Chemistry Unit, Technology Research Laboratory, Shimadzu Corporation, Kyoto, Japan
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