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Shimadzu Review 80[3・4] (2023)
Abstract
Gut bacteria (flora) have recently been shown to affect human health in a variety of ways and there is growing interest in gut bacteria-oriented methods of improving health and preventing and treating disease. While assessing the interaction between gut bacteria and host (cells) is an important step in utilizing gut bacteria, the majority of gut bacteria are anaerobes intolerant of oxygen exposure and require an anaerobic environment not survivable by human cells. Because of this incompatibility, conventional in vitro experimental systems are unable to co-culture human cells and gut bacteria in a viable state. We developed the gut microbial co-culture device consisting of a novel in vitro experimental system with an anaerobic chamber and flow-based media supply system that enables the co-culture of gut bacteria and human cells. This article provides an overview of the device and example applications of the device with human iPS cell-derived intestinal epithelial cells.
1Biology･Chemistry Unit, Technology Research Laboratory, Shimadzu Corporation, Kyoto, Japan
2Business Development Department, Technology Research Laboratory, Shimadzu Corporation, Kyoto, Japan
3Research Promotion Department, Technology Research Laboratory, Shimadzu Corporation, Kyoto, Japan
4Life Science Research Center, Technology Research Laboratory, Shimadzu Corporation, Kyoto, Japan
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