Preparative Purification Liquid Chromatograph
This kit enables large-capacity injections of 2 mL or more at one time. The maximum injection volume is 20 mL.
This reduces the washing time for the injection needle, increasing throughput while reducing carryover.
The recovered fraction can be reanalyzed to check the purity.
This detects the liquid surface level, and automatically determines whether there is any sample present. As a result, only the remaining volume is injected, which prevents the injection of air into columns. Additionally, if no sample is present, the system can proceed to the next sample, reducing needless errors and lost labor.
Up to six LH-40 liquid handlers can be connected, making it easy to inject the sample from all LH-40. *1 Up to one LH-40 when FRC-40 fraction collectors are connected.
Autosampler
Optional Syringe Unit
Sample Racks
Block Cooling/Heating: 4 to 70 °C)
Manual Injector
Optional Sample Loops (Material: SUS)
| Volume | Part Number(P/N) |
|---|---|
| 100 μL | 228-32211-16 |
| 200 μL | 228-32211-17 |
| 500 μL | 228-32211-18 |
| 1 mL | 228-32211-19 |
| 5 mL | 228-32211-21 |
| LH-40 | SIL-40/SIL-40C | SIL-10AP | |
|---|---|---|---|
| Maximum Injection Volume | Up to 2 mL, up to 20 mL (Optional available) | Up to 2 mL | Up to 5 mL |
| Cooling Function | None | SIL-40: None SIL-40C: 4 to 45 °C (air temperature controlling system) |
Yes (Optionally available) |
| Compatible Containers and Quantities |
10 mmO.D. test tube 540 pcs 1.5 mL vial 486 pcs*2
12 mmO.D. test tube 486 pcs 4.0 mL vial 252 pcs
13 mmO.D. test tube 360 pcs 13 mL vial 108 pcs
15 mmO.D. test tube 252 pcs 50 mL vial 54 pcs
17, 18 mmO.D. test tube 216 pcs 250 mL bottle 20 pcs
25 mmO.D. test tube 108 pcs 500, 1000 mL bottle 12 pcs
30, 35 mmO.D. test tube 54 pcs 96 well MTP/DWP 9 pcs
|
288 (using microplates, 96 wells × 3 plates) 1152 (using microplates, 384 wells × 3 plates) 252 (using 1 mL sample vial plates, 84 vials × 3 plates) 162 (using 1.5 mL sample vial plates, 54 vials × 3 plates) 84 (using 4 mL sample vial plates, 28 vials × 3 plates) 36 (using 10 mL sample vial plates, 12 vials × 3 plates) 72 (using 1.5 mL microtube plates, 24 microtubes × 3 plates) |
1.5 mL vials 100 pcs
4.0 mL vials 80 pcs
13 mL vials 25 pcs
|
| Compatible Containers and Quantities | W 390 x H 560 x D 730 mm, 40 kg | W 260 × H 280 × D 500 mm (With the SIL-40C, the protrusion depth adds 140 mm) SIL-40: 17 kg; SIL-40C: 24 kg |
W 260 x H 280 x D 320 mm, 18.5 kg |
*2 Available for injection sample container. Not available for fraction container.
A variety of containers can be placed including MTPs, vials, and various types of test tubes. Six colors are available, so a separate color can be apportioned to each user in order to avoid confusing samples.
Up to six FRC-40 fraction collectors (up to five LH-40 liquid handlers) can be connected, making it easy to increase the number of fractions.
For smaller scale collection, or specialized applications that require enclosure and cooling, the FRC-10A is a compact fraction collector that provides time and signal based triggering. A variety of programmable fractionation functions enable target components to be collected with high purity and high recovery.。
| FRC-40 | FRC-10A | |
|---|---|---|
| Maximum Flow Rate | 150 mL/min | |
| Fractionation Mode | Configured through a combination of basic mode (initial parameter mode) and time program mode (14 parameters) | |
| Cooling Function | None | Yes (Optionally available) |
| Compatible Containers and Quantities |
10 mmO.D. test tube 540 pcs 4 mL vial 252 pcs
12 mmO.D. test tube 486 pcs 15 mL vial 108 pcs 13 mmO.D. test tube 360 pcs 50 mL vial 54 pcs
15 mmO.D. test tube 252 pcs 250 mL vial 20 pcs 17, 18, mmO.D. test tube 216 pcs 500, 1000 mL vial 12 pcs 25 mmO.D. test tube 108 pcs 96 well MTP/DWP 9 pcs 30, 35 mmO.D. test tube 54 pcs |
10 mmO.D. test tubes 144 pcs
18 mmO.D. test tubes 164 pcs 35 mmO.D. test tubes 16 pcs |
| Size and weight | W 390 × H 560 × D 730 mm, 30 kg W 390 × H 865 × D 730 mm; 43 kg (when an optional hood is attached) |
W 260 x H 280 x D 320 mm，18.5 kg |
This is an example of scaling up in which seven types of parabens are targeted using a 150 mm long column with a particle size of 5 μm.
The gradient elution conditions investigated at the analytical scale are transitioned to the semi-preparative scale. A comparable chromatogram is obtained at both scales.
Column: Shim-pack Scepter C18-120 (4.6 mm x 150 mm，5 μm）
Column: Shim-pack Scepter C18-120 (20 mm x 150 mm，5 μm）
Preparative columns with an I.D. of 20 mm to 50 mm as well as manual switching valves can be attached. The valves can be used for column switching.
| Installable Valves | Installable Columns | Size | |
|---|---|---|---|
| Column Hub | Automatic Switching Valves Up to 4 pcs | Preparative Columns 2 pcs Analytical Columns 6 pcs* | W 260 x H 560 x D 500 mm |
| Column Hub + Column Hub Kit | Automatic switching valves, up to 4 (manual switching valves/manual injectors not allowed) |
I.D. 2.1 to 30 mm columns, 6 pc. | |
| Column Holder | Manual Switching Valves Up to 4 pcs | I.D. 20 to 50 mm columns, 2 pc. | W 250 x H 465 x D 400 mm |
| Column Holder SLIM | Manual Switching Valves Up to 5 pcs | I.D. 2.1 to 30 mm columns, 1 pc. + I.D. 20 to 50 mm columns, 2 pc. |
W 110 x H 625 x D 500 mm |
This is required for preparative work using multiple detector triggers. Expand the hardware to suit the number of detection trigger channels required.
* When connecting to an LC-20AP, a connection kit must be obtained separately.
* LC-20AR connection kit is required when the operating flow rate is more than 10 mL/min.
