UV-2600i Plus/UV-2700i Plus - Applications

The diffuse reflection spectra for two types of compound semiconductors (red line: Culn_0.5Ga_0.5Se₂, blue line: CulnSe₂) used as solar cell materials were measured using the ISR-2600Plus integrating sphere. It is evident that the absorption edge (position where the reflectance drops) differs depending on the sample. This difference signifies a difference in the band gap* for these samples. (The samples were provided by Wada Laboratory, Faculty of Science and Technology, Ryukoku University.) The band gaps for the samples were calculated utilizing the Tauc method. The results obtained were 1.27 eV for Culn_0.5Ga_0.5Se₂ (red line) and 0.99 eV for CulnSe₂ (blue line). * The term band gap refers to the energy difference between the top of the valence band, which is full of electrons, and the bottom of the conduction band, which does not contain electrons.

Undulating interference waveforms sometimes occur if light is passed through a film. The film thickness of a sample can be determined by using these interference waveforms. The figure shows transmittance data for a cling film. A clear interference waveform is produced. Utilizing optional film thickness measurement software, the film thickness is calculated as 10.4 μm. (Caution) The sample's refractive index must be entered for the film thickness calculation.

Oligonucleotide therapeutics samples (9 to 60 M) were analyzed using TMSPC™-8i and 1 mm optical path length cells. Since UV-2600i Plus /2700i Plus can measure over a wide dynamic range, it is possible to perform a thermal stability analysis of nucleic acid drugs by obtaining the Tm value from the melting curve.

Two types of window glass were measured utilizing the ISR-2600Plus integrating sphere. The sample shown by the red line is highly transparent to near-infrared light at 800 nm or more. The sample shown by the blue line, however, is apparently not very transparent to near-infrared light.

The UV protection provided by clothes and an umbrella were analyzed by an ISR-2600Plus integrating sphere accessory. The transmittance spectrum of the umbrella (green) shows that very little UV radiation transmits through the umbrella. In regards to the clothing, the black areas block more UV radiation than do the white areas.

An ISR-2600Plus integrating sphere was used to measure the total transmittance spectrum of cellulose nanofiber (CNF). Depending on the raw materials used, spectra can vary, transparency can vary, and spectral tendencies in the ultraviolet region can vary.

The red and blue lines are the absorption spectra for dsDNA and BSA (bovine serum albumin), respectively.
The concentration values are 45 ng/μL for dsDNA and 2.2 mg/mL for BSA.

This shows the absorption spectra for riboflavin (vitamin B₂).
The sample concentrations are, in order from the highest absorbance, 0.08,
0.04, 0.02, and 0.01 mg/mL.